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Sodium Thioglycollate 80% For Bacteriology 500 gr

SKU : O-5271.0500
CAS : 367-51-1
Formula : C2H3NaO2S
Grade : For Bacteriology
Applications 
  • Cultivating Anaerobes:
    Essential for growing and isolating anaerobic bacteria from various sources, such as environmental samples and bodily fluids.
  • Sterility Testing:
    Used for the sterility testing of antibiotics, biological products, and foods that may contain turbid or viscous components.
  • Isolating Strict Anaerobes:
    Employed in blood cultures to isolate strict anaerobic bacteria during suspected infections.
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Sodium thioglycollate is used in bacteriology as a reducing agent in Fluid Thioglycollate Medium, a type of broth that establishes anaerobic conditions to promote the growth of oxygen-sensitive (anaerobic) bacteria. It works by reacting with dissolved oxygen, lowering the oxygen concentration within the medium. This creates a gradient, allowing for the simultaneous cultivation and differentiation of aerobic, facultative anaerobic, and obligate anaerobic bacteria based on their oxygen requirements. The concentration of oxygen at different levels of the broth can be visualized using a redox-sensitive dye. 
How it works in Thioglycollate Medium 
  • Reduces Oxygen:
    Sodium thioglycollate chemically binds with dissolved oxygen, creating an anaerobic environment at the bottom of the tube.
  • Creates an Oxygen Gradient:
    Oxygen diffusion from the top of the broth, combined with the oxygen-reducing action of thioglycollate, forms a range of oxygen levels from high at the surface to near-zero at the bottom.
  • Differentiates Bacteria:
    • Obligate aerobes: (require oxygen) grow at the top of the tube.
    • Obligate anaerobes: (oxygen-intolerant) grow at the bottom.
    • Facultative anaerobes: (can grow with or without oxygen) grow in the upper regions where some oxygen is present.
  • Uses Redox Indicators:
    A redox dye, like resazurin, is often included in the medium. It turns pink in the presence of oxygen, providing a visual indicator of the oxygen concentration at different depths.

TINDAKAN PENCEGAHAN UNTUK PENANGANAN YANG AMAN

Untuk tindakan pencegahan lihat bagian 2.2 dalam SDS.

KONDISI PENYIMPANAN YANG AMAN, TERMASUK KETIDAKCOCOKAN

a. Kondisi penyimpanan

Wadah yang tidak mengandung logam. Lindungi dari cahaya.Tertutup sangat rapat. Kering. Simpan di tempat yang berventilasi baik. Simpan dalam tempat terkunci atau di tempat yang hanya bisa dimasuki oleh orangorang yang mempunyai kualifikasi atau berwenang.

Suhu penyimpanan : Rekomendasi suhu penyimpanan lihat pada label

b. Kelas penyimpanan

Kelas penyimpanan Jerman (TRGS 510): 6.1C: Kat.3 toksik akut, mudah terbakar / senyawa toksik atau senyawa yang menyebabkan efek-efek kronis.

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