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Masson’s Trichrome Staining: This is its most legendary use in pathology and anatomy. Aniline Blue serves as the final counterstain in trichrome protocols to visually differentiate tissues. It binds specifically to collagen (the main structural protein in connective tissue), turning it a brilliant, deep blue. This creates a sharp contrast against muscle fibers and cytoplasm (which stain red) and cell nuclei (which stain black/purple). This layout is vital for diagnosing medical conditions like liver cirrhosis or kidney fibrosis, where tracking excess scar tissue (collagen accumulation) is key.
Plant Histology: In botany, Aniline Blue is paired with other stains to highlight plant structures. It features a high affinity for callose—a carbohydrate structure found in pollen tubes and around sieve plates in the plant’s phloem (the tissue that transports sugars)—helping researchers track plant reproductive and vascular development.
Fluorescence Microscopy: Interestingly, Aniline Blue can function as a fluorochrome. When exposed to ultraviolet (UV) light, it fluoresces a bright yellow-green. Scientists use this property to detect fungal cell walls (which contain chitin and glucans) infecting plant or animal tissues.
Aniline Blue (Water soluble, C.I. 42780) for microscopy is a highly purified acidic triarylmethane biological dye primarily used as a contrast and connective tissue stain in histology and cytology. It is also commonly referred to as Methyl Blue or Acid Blue 93.
Aniline Blue (Water soluble) (C.I. 42780) for microscopy is a specialized biological dye used primarily in histology (the study of tissue anatomy) and cytology to stain specific structural components of cells and tissues for microscopic examination.
The “C.I. 42780” designation refers to its unique Color Index number, which standardizes its chemical identity worldwide, distinguishing it from alcohol-soluble variants or other blue dyes.
TINDAKAN PENCEGAHAN UNTUK PENANGANAN YANG AMAN
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KONDISI PENYIMPANAN YANG AMAN, TERMASUK KETIDAKCOCOKAN
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b. Kelas penyimpanan
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